Blood Cell Counters - Basics
Lab Equipment Orientation Index
What does it do?
Counts the number of each type of cell found in whole blood.
Physiology
Blood is made up of white blood cells (WBC), Red blood cells (RBC), Platelets (PLT) and Haemoglobin (Hb).

How it works

Blood is collected from the patient and put into a test tube. The test tube is bar coded and put into the analyser for measurement. The WBC, RBC and PLT’s are counted using the “Coulter principle” where the resistance of a circuit changes as the cells pass through a narrow aperture.
RBC’s, WBC’s and PLT’s are different sizes. The number of resistance changes and the amount of each change determine the number and size (hence type) of cells.
The Hb is measured using a photometer at a wavelength specific for Hb. Results are transmitted to the Laboratory Information System or may be printed out.

Units of measurement RBC, WBC, PLT are expressed in counts per micro litre (µL)
Hb may be grams per litre (g/L)

Typical values

WBC – 10.0 x 10^9/L
RBC – 4.00 x 10^12/L
PLT – 200 x 10^9/L
Hb   – 130g/L
Picture of equipment
Coulter cell counter

Updated: July 14, 2006